Information-enriched characterization of cell populations. Morphological information and facts from illustrations or photos can incorporate towards the richness of flow cytometry data. By way of example, the figure reveals an or else traditional apoptosis assay applying Annexin V and PI, incorporating cell imaging to characterize cells in Each and every population to expose morphologically distinct features. These insights couldn't are actually acquired from multiplex staining on your own.

As samples are acquired around the Attune CytPix Flow Cytometer, the large-speed brightfield digicam captures and shops illustrations or photos of detected functions in a price up to six,000 images for each 2nd, dependant upon the flow fee and picture sizing. For bigger flexibility, Attune Cytometric Computer software lets you modify the image capture frequency as wanted and select a specific gate for image seize, if demanded.

(B) Uncooked 264.7 macrophages are often annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The volume of singlets available for Examination improves once the person trains and employs a far more correct product.

The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating with a significant-speed brightfield digicam for simultaneous higher throughput flow cytometry and significant resolution brightfield imaging.

*Pricing is indicative only and is matter to vary. Be sure to Speak to your Agilent agent with any concerns. The data set forth herein is not really a suggestion or possibly a commitment, both equally of that are subject matter to credit rating acceptance, conditions and terms, and availability with Agilent. Agilent Money Alternatives is available in picked nations.

In this article, a qualified consumer has gated singlets confidently. Right after evaluating the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate includes more than four% aggregates.

Optimizing gating system. Even sturdy manual singlet gating is error-vulnerable and continues to be a subjective final decision position in Nearly all flow cytometry assays. Imaging can be utilized to confirm and alter gates to include only one cells of interest.

Our experts will style panels for yourself as persistently you may need—free of charge, no product or service buy needed.

The Attune NxT cytometer is definitely the trusted workhorse instrument your lab can depend on for dependable and dependable effectiveness. The Attune CytPix model contains a superior-pace brightfield digicam and AI-pushed image Examination application, with new updates which include equipment Studying resources and an intuitive user interface, enabling Increased info precision, precision, and quicker insights into sample biology.

Buyers can view celebration visuals in real-time to confirm sample top quality, improve protocols, and document the morphology of every mobile populace identified for Examination. Imaging capture also Added benefits from acoustic concentrating, which really helps to placement the cells to make sure that a sharp, centered picture is obtained.

Seem waves help exact mobile alignment, causing Outstanding details quality and ~10X more quickly sample flow rates.

Application Take note: Automated graphic Investigation lessens consumer-to-user variability in flow cytometry gating approaches

Leverage the power of Highly developed engineering and innovation to accelerate your discoveries. Belief inside the Attune Flow Cytometer relatives to deliver the trustworthiness, pace, and accuracy that set your do the job aside.

No wash/no lyse leukocyte Assessment with imaging. Cells had been obtained from check here 24-hour-previous blood by dilution in 1 mM EDTA ( Skilled UX/UI enables consumers to prepare AI models for expanded programs. (A) The “Educate Design” element supplies annotation tools and true-time design teaching precision.

Gating on these extended parameters lets you promptly and precisely discover populations of interest and make sure gating strategy with little or no handbook evaluate. The Attune software program gives entry to state-of-the-artwork machine Discovering Examination resources inside an expertly designed consumer interface, which makes it exceptionally easy to use.

Since the mobile line had Formerly been analyzed over the Attune CytPix Flow Cytometer, the researchers went back to the images and were being capable to document the microbial an infection no less than 5 times previously.

Agilent Worth Guarantee assures you not less than ten years of instrument use through the day you purchase. Or else, We're going to credit you Along with the residual value of the method toward an upgraded product.

Right here, we display that by gating methods using image-based quantitative parameters can distinguish interacting cells from coincident events to a lot more precisely assess interacting cells.